› 7yk3

X-ray diffraction
2.2Å resolution

Crystal structure of DarTG toxin-antitoxin complex from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb7yk3/pdb
Model geometry
Fit model/data
PDB entry 7yk3 coloured by chain, front view.
PDB entry 7yk3 coloured by chain, side view.
PDB entry 7yk3 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural insights into DarT toxin neutralization by cognate DarG antitoxin: ssDNA mimicry by DarG C-terminal domain keeps the DarT toxin inhibited.
Deep A, Singh L, Kaur J, Velusamy M, Bhardwaj P, Singh R, Thakur KG
Structure (2023)
PMID: 37167974

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216665 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA ADP-ribosyl transferase Chains: A, C
Molecule details ›
Chains: A, C
Length: 242 amino acids
Theoretical weight: 26.84 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: O53604 (Residues: 1-230; Coverage: 100%)
Gene names: Rv0059, darT
Sequence domains: ssDNA thymidine ADP-ribosyltransferase, DarT
DNA ADP-ribosyl glycohydrolase Chains: B, D
Molecule details ›
Chains: B, D
Length: 189 amino acids
Theoretical weight: 20.84 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O53605 (Residues: 164-352; Coverage: 54%)
Gene names: Rv0060, darG

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ELETTRA BEAMLINE 11.2C
Spacegroup: P1
Unit cell:
a: 40.53Å b: 54.55Å c: 93.09Å
α: 105.03° β: 93.1° γ: 102.69°
R-values:
R R work R free
0.182 0.179 0.25
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Deregulated DNA ADP-ribosylation impairs telomere replication.
Wondisford et al. (2024)
3 more