› 7yrt

X-ray diffraction
2.27Å resolution

Crystal structure of PAS like domain of FlrB, the histidine kinase involved in flagellar synthesis of Vibrio cholerae

Released:
DOI: 10.2210/pdb7yrt/pdb
PDB entry 7yrt coloured by chain, front view.
PDB entry 7yrt coloured by chain, side view.
PDB entry 7yrt coloured by chain, top view.
Source organism: Vibrio cholerae O395
Primary publication:
PAS domain of flagellar histidine kinase FlrB has a unique architecture and binds heme as a sensory ligand in an unconventional fashion.
Mukherjee P, Agarwal S, Mallick SB, Dasgupta J
Structure 32 200-216.e5 (2024)
PMID: 38157857

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 138 amino acids
Theoretical weight: 15.54 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: H9L4P6 (Residues: 7-123; Coverage: 33%)
Gene name: VC_2136
Sequence domains: PAS domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 212.644Å b: 49.556Å c: 146.83Å
α: 90° β: 92.53° γ: 90°
R-values:
R R work R free
0.188 0.185 0.251
Expression system: Escherichia coli BL21

Quick links