› 7z0w

X-ray diffraction
2.06Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxygen-insensitive NADPH nitroreductase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 240 amino acids
Theoretical weight: 26.83 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17117 (Residues: 1-240; Coverage: 100%)
Gene names: JW0835, b0851, mda18, mdaA, nfsA, ybjB
Sequence domains: Nitroreductase family

Ligands and Environments

3 bound ligands:
Ligand FMN 8 x FMN
Ligand ATR 2 x ATR
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 96.766Å b: 110.757Å c: 112.25Å
α: 90° β: 103.84° γ: 90°
R-values:
R R work R free
0.155 0.153 0.187
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Structure and Dynamics of Three Escherichia coli NfsB Nitro-Reductase Mutants Selected for Enhanced Activity with the Cancer Prodrug CB1954.
Day et al. (2023)