› 7z2v

X-ray diffraction
1.45Å resolution

Ferulic acid esterase variant S114A from Lactobacillus buchneri

Released:
DOI: 10.2210/pdb7z2v/pdb
PDB entry 7z2v coloured by chain, front view.
PDB entry 7z2v coloured by chain, side view.
PDB entry 7z2v coloured by chain, top view.
Source organism: Lentilactobacillus buchneri
Primary publication:
Crystal structure of the feruloyl esterase from Lentilactobacillus buchneri reveals a novel homodimeric state.
Kasmaei KM, Kalyani DC, Reichenbach T, Jiménez-Quero A, Vilaplana F, Divne C
Front Microbiol 13 1050160 (2022)
PMID: 36569051

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine aminopeptidase S33 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 31.67 KDa
Source organism: Lentilactobacillus buchneri
Expression system: Escherichia coli B
UniProt:
  • Canonical: D7RU28 (Residues: 1-260; Coverage: 100%)
Gene name: faeA
Sequence domains: Alpha/beta hydrolase family

Ligands and Environments

2 bound ligands:
Ligand CA 5 x CA
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 79.96Å b: 79.96Å c: 227.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.177
Expression system: Escherichia coli B

Quick links

Citations

1 citations in other articles

Comparison of metagenomes from fermentation of various agroindustrial residues suggests a common model of community organization.
Myers et al. (2023)

1 mention without citation

Crystal structure of the feruloyl esterase from Lentilactobacillus buchneri reveals a novel homodimeric state.
Kasmaei et al. (2022)