› 7z7e

X-ray diffraction
1.8Å resolution

Crystal structure of p63 DNA binding domain in complex with inhibitory DARPin G4

Released:
DOI: 10.2210/pdb7z7e/pdb
Model geometry
Fit model/data
PDB entry 7z7e coloured by chain, front view.
PDB entry 7z7e coloured by chain, side view.
PDB entry 7z7e coloured by chain, top view.
Source organisms:
Primary publication:
Designed Ankyrin Repeat Proteins as a tool box for analyzing p63.
Strubel A, Münick P, Chaikuad A, Dreier B, Schaefer J, Gebel J, Osterburg C, Tuppi M, Schäfer B, Knapp S, Plückthun A, Dötsch V
OpenAccess logo Cell Death Differ (2022)
PMID: 35717504

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274292 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tumor protein 63 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 22.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9H3D4 (Residues: 162-363; Coverage: 30%)
Gene names: KET, P63, P73H, P73L, TP63, TP73L
Sequence domains: P53 DNA-binding domain
DARPIN Chain: B
Molecule details ›
Chain: B
Length: 159 amino acids
Theoretical weight: 16.91 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 96.578Å b: 96.578Å c: 77.071Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.197 0.249
Expression system: Escherichia coli BL21

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Citations

2 review citations

p73 isoforms meet evolution of metastasis.
Logotheti et al. (2022)
1 more