7z8i

Electron Microscopy
3.3Å resolution

The barbed end complex of dynactin bound to BICDR1 and the cytoplasmic dynein tails (A2, B1, B2)

Released:
Related structures: EMD-14552

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptadecamer (preferred)
PDBe Complex ID:
PDB-CPX-105232 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Alpha-centractin Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 376 amino acids
Theoretical weight: 42.67 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F2Z5G5 (Residues: 1-310; Coverage: 89%)
Gene name: ACTR1A
Sequence domains: Actin
F-actin-capping protein subunit alpha Chain: K
Molecule details ›
Chain: K
Length: 286 amino acids
Theoretical weight: 33.06 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1T8I3 (Residues: 1-286; Coverage: 100%)
Gene name: CAPZA1
Sequence domains: F-actin capping protein alpha subunit
F-actin-capping protein subunit beta Chain: L
Molecule details ›
Chain: L
Length: 272 amino acids
Theoretical weight: 30.67 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0PFK7 (Residues: 1-272; Coverage: 100%)
Gene name: CAPZB
Sequence domains: F-actin capping protein, beta subunit
BICD family-like cargo adapter 1 Chains: X, x
Molecule details ›
Chains: X, x
Length: 577 amino acids
Theoretical weight: 65.38 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0JNT9 (Residues: 1-577; Coverage: 100%)
Gene names: Bicdl1, Bicdr1, Ccdc64
Cytoplasmic dynein 1 intermediate chain 2 Chains: h, o
Molecule details ›
Chains: h, o
Length: 638 amino acids
Theoretical weight: 71.55 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13409 (Residues: 1-638; Coverage: 100%)
Gene names: DNCI2, DNCIC2, DYNC1I2
Sequence domains:
Cytoplasmic dynein 1 light intermediate chain 2 Chains: j, r
Molecule details ›
Chains: j, r
Length: 492 amino acids
Theoretical weight: 54.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43237 (Residues: 1-492; Coverage: 100%)
Gene names: DNCLI2, DYNC1LI2, LIC2
Sequence domains: Dynein light intermediate chain (DLIC)

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-14552
Expression system: Spodoptera frugiperda