› 7zh9

X-ray diffraction
1.72Å resolution

Uba1 in complex with ATP

Released:
DOI: 10.2210/pdb7zh9/pdb
PDB entry 7zh9 coloured by chain, front view.
PDB entry 7zh9 coloured by chain, side view.
PDB entry 7zh9 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of UBA6 explain its dual specificity for ubiquitin and FAT10.
Truongvan N, Li S, Misra M, Kuhn M, Schindelin H
Nat Commun 13 4789 (2022)
PMID: 35970836

Function and Biology Details

Reaction catalysed: 
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-activating enzyme E1 1 Chain: A
Molecule details ›
Chain: A
Length: 1024 amino acids
Theoretical weight: 114.39 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P22515 (Residues: 1-1024; Coverage: 100%)
Gene names: UBA1, YKL210W
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand ATP 1 x ATP
Ligand SO4 1 x SO4
Ligand ACT 1 x ACT
Ligand MG 3 x MG
Ligand GOL 10 x GOL
Ligand K 1 x K
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 107.72Å b: 118.064Å c: 196.654Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.206
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Molecular basis for ubiquitin/Fubi cross-reactivity in USP16 and USP36.
O'Dea et al. (2023)
5 more