› 7ziz

X-ray diffraction
1.5Å resolution

X-ray structure of the dead variant haloalkane dehalogenase HaloTag7-D106A bound to a pentanol tetramethylrhodamine ligand (TMR-Hy5)

Released:
DOI: 10.2210/pdb7ziz/pdb
PDB entry 7ziz coloured by chain, front view.
PDB entry 7ziz coloured by chain, side view.
PDB entry 7ziz coloured by chain, top view.
Source organism: Rhodococcus sp.
Primary publication:
Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy.
Kompa J, Bruins J, Glogger M, Wilhelm J, Frei MS, Tarnawski M, D'Este E, Heilemann M, Hiblot J, Johnsson K
J Am Chem Soc 145 3075-3083 (2023)
PMID: 36716211

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33.18 KDa
Source organism: Rhodococcus sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A3G3 (Residues: 4-293; Coverage: 99%)
Gene name: dhaA
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

3 bound ligands:
Ligand IYL 1 x IYL
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21212
Unit cell:
a: 77.83Å b: 88.71Å c: 44.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.195
Expression system: Escherichia coli

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Citations

6 review citations

Beyond DNA: new probes for PAINT super-resolution microscopy.
Tholen et al. (2023)
5 more

1 mention without citation

Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy.
Kompa et al. (2023)