› 7zqs

Electron Microscopy
2.54Å resolution

Cryo-EM Structure of Human Transferrin Receptor 1 bound to DNA Aptamer

Released:
DOI: 10.2210/pdb7zqs/pdb
PDB entry 7zqs coloured by chain, front view.
PDB entry 7zqs coloured by chain, side view.
PDB entry 7zqs coloured by chain, top view.
Source organisms:
Primary publication:
Discovery of a Transferrin Receptor 1-Binding Aptamer and Its Application in Cancer Cell Depletion for Adoptive T-Cell Therapy Manufacturing.
Cheng EL, Cardle II, Kacherovsky N, Bansia H, Wang T, Zhou Y, Raman J, Yen A, Gutierrez D, Salipante SJ, des Georges A, Jensen MC, Pun SH
J Am Chem Soc 144 13851-13864 (2022)
PMID: 35875870
Related structures: EMD-14874

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122425 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (3 distinct):
Transferrin receptor protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 760 amino acids
Theoretical weight: 84.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P02786 (Residues: 1-760; Coverage: 100%)
Gene name: TFRC
Sequence domains:
DNA (30-MER) Chains: A, C
Molecule details ›
Chains: A, C
Length: 51 nucleotides
Theoretical weight: 15.99 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand MG 4 x MG
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.54Å
Relevant EMDB volumes: EMD-14874
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

3 review citations

Aptamers 101: aptamer discovery and in vitro applications in biosensors and separations.
Yang et al. (2023)
2 more

1 mention without citation

Characterization of a fatal feline panleukopenia virus derived from giant panda with broad cell tropism and zoonotic potential.
Zhao et al. (2023)