› 7zr3

X-ray diffraction
2.01Å resolution

STRUCTURE OF ESTER-HYDROLASE EH0 FROM THE METAGENOME OF SORGHUM BICOLOR RHIZOSPHERE FROM THE HENFAES RESEARCH CENTRE (GWYNEDD, WALES)

Released:
DOI: 10.2210/pdb7zr3/pdb
PDB entry 7zr3 coloured by chain, front view.
PDB entry 7zr3 coloured by chain, side view.
PDB entry 7zr3 coloured by chain, top view.
Source organism: metagenome
Primary publication:
The Mobility of the Cap Domain Is Essential for the Substrate Promiscuity of a Family IV Esterase from Sorghum Rhizosphere Microbiome.
Distaso M, Cea-Rama I, Coscolín C, Chernikova TN, Tran H, Ferrer M, Sanz-Aparicio J, Golyshin PN
Appl Environ Microbiol 89 e0180722 (2023)
PMID: 36602332

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222096 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 36.75 KDa
Source organism: metagenome
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A8E4HNI8 (Residues: 1-324; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

4 bound ligands:
Ligand ACT 2 x ACT
Ligand CL 1 x CL
Ligand GOL 4 x GOL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 63.958Å b: 116.666Å c: 128.557Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Enhancing the Hydrolytic Activity of a Lipase towards Larger Triglycerides through Lid Domain Engineering.
Fernandez-Lopez et al. (2023)
2 more

1 mention without citation

The Mobility of the Cap Domain Is Essential for the Substrate Promiscuity of a Family IV Esterase from Sorghum Rhizosphere Microbiome.
Distaso et al. (2023)