› 7zub

Electron Microscopy
2.85Å resolution

Cryo-EM structure of the indirubin-bound Hsp90-XAP2-AHR complex

Released:
DOI: 10.2210/pdb7zub/pdb
PDB entry 7zub coloured by chain, front view.
PDB entry 7zub coloured by chain, side view.
PDB entry 7zub coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the agonist-bound Hsp90-XAP2-AHR cytosolic complex.
Gruszczyk J, Grandvuillemin L, Lai-Kee-Him J, Paloni M, Savva CG, Germain P, Grimaldi M, Boulahtouf A, Kwong HS, Bous J, Ancelin A, Bechara C, Barducci A, Balaguer P, Bourguet W
OpenAccess logo Nat Commun 13 7010 (2022)
PMID: 36385050
Related structures: EMD-14971

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-271444 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Heat shock protein HSP 90-beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 730 amino acids
Theoretical weight: 84.21 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08238 (Residues: 2-724; Coverage: 100%)
Gene names: HSP90AB1, HSP90B, HSPC2, HSPC3, HSPCB
Sequence domains:
AH receptor-interacting protein Chain: C
Molecule details ›
Chain: C
Length: 330 amino acids
Theoretical weight: 37.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O00170 (Residues: 1-330; Coverage: 100%)
Gene names: AIP, XAP2
Sequence domains: FKBP-type peptidyl-prolyl cis-trans isomerase
Aryl hydrocarbon receptor Chain: D

Ligands and Environments

4 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
Ligand MOO 2 x MOO
Ligand JY6 1 x JY6
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.85Å
Relevant EMDB volumes: EMD-14971
Expression system: Spodoptera frugiperda

Quick links

Citations

8 review citations

The complex biology of aryl hydrocarbon receptor activation in cancer and beyond.
Opitz et al. (2023)
7 more

5 mentions without citation

Dimerization Rules of Mammalian PAS Proteins.
Rojas et al. (2024)
4 more