› 7zvp

X-ray diffraction
1.61Å resolution

Crystal structure of poplar glutathione transferase U19 in complex with glutathione

Released:
DOI: 10.2210/pdb7zvp/pdb
PDB entry 7zvp coloured by chain, front view.
PDB entry 7zvp coloured by chain, side view.
PDB entry 7zvp coloured by chain, top view.
Source organism: Populus trichocarpa
Primary publication:
Biochemical and Structural Insights on the Poplar Tau Glutathione Transferase GSTU19 and 20 Paralogs Binding Flavonoids.
Sylvestre-Gonon E, Morette L, Viloria M, Mathiot S, Boutilliat A, Favier F, Rouhier N, Didierjean C, Hecker A
Front Mol Biosci 9 958586 (2022)
PMID: 36032685

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110823 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 25.05 KDa
Source organism: Populus trichocarpa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9HKX9 (Residues: 1-219; Coverage: 100%)
Gene name: POPTR_008G174900
Sequence domains:

Ligands and Environments


Cofactor: Ligand GS8 1 x GS8
1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P41212
Unit cell:
a: 56.727Å b: 56.727Å c: 182.015Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.229 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Biochemical and Structural Characterization of Chi-Class Glutathione Transferases: A Snapshot on the Glutathione Transferase Encoded by sll0067 Gene in the Cyanobacterium Synechocystis sp. Strain PCC 6803.
Mocchetti et al. (2022)

1 mention without citation

Biochemical and Structural Insights on the Poplar Tau Glutathione Transferase GSTU19 and 20 Paralogs Binding Flavonoids.
Sylvestre-Gonon et al. (2022)