› 8a2c

X-ray diffraction
1.6Å resolution

The crystal structure of the S178A mutant of PET40, a PETase enzyme from an unclassified Amycolatopsis

Released:
DOI: 10.2210/pdb8a2c/pdb
PDB entry 8a2c coloured by chain, front view.
PDB entry 8a2c coloured by chain, side view.
PDB entry 8a2c coloured by chain, top view.
Source organism: unclassified Amycolatopsis
Entry authors: Costanzi E, Applegate V, Port A, Smits SHJ

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217658 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PET40 S178A Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 29.6 KDa
Source organism: unclassified Amycolatopsis
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

4 bound ligands:
Ligand EPE 2 x EPE
Ligand EDO 5 x EDO
Ligand CL 10 x CL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 46.038Å b: 110.002Å c: 47.962Å
α: 90° β: 95.74° γ: 90°
R-values:
R R work R free
0.148 0.146 0.179
Expression system: Escherichia coli BL21(DE3)

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