› 8af4

X-ray diffraction
1.51Å resolution

Room temperature SSX structure of GH11 xylanase from Nectria haematococca (40000 frames)

Released:
DOI: 10.2210/pdb8af4/pdb
Model geometry
Fit model/data
PDB entry 8af4 coloured by chain, front view.
PDB entry 8af4 coloured by chain, side view.
PDB entry 8af4 coloured by chain, top view.
Source organism: Fusarium haematococcum
Primary publication:
Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.
Zielinski KA, Prester A, Andaleeb H, Bui S, Yefanov O, Catapano L, Henkel A, Wiedorn MO, Lorbeer O, Crosas E, Meyer J, Mariani V, Domaracky M, White TA, Fleckenstein H, Sarrou I, Werner N, Betzel C, Rohde H, Aepfelbacher M, Chapman HN, Perbandt M, Steiner RA, Oberthuer D
OpenAccess logo IUCrJ 9 778-791 (2022)
PMID: 36381150

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111784 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 20.58 KDa
Source organism: Fusarium haematococcum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C7YSL3 (Residues: 34-222; Coverage: 93%)
Gene name: NECHADRAFT_106153
Sequence domains: Glycosyl hydrolases family 11

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: C2
Unit cell:
a: 80.55Å b: 38.85Å c: 53.57Å
α: 90° β: 91° γ: 90°
R-values:
R R work R free
0.154 0.152 0.183
Expression system: Escherichia coli BL21(DE3)

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Citations

8 citation in other articles

JINXED: just in time crystallization for easy structure determination of biological macromolecules.
Henkel et al. (2023)
7 more