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8aum

X-ray diffraction
1.35Å resolution

OPR3 Y190F variant in complex with ethyl (Z)-2-(hydroxyimino)-3-oxopentanoate

Released:
Model geometry
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Function and Biology Details

Reaction catalysed:
(1S,2S)-OPC-8 + NADP(+) = (9S,13S,15Z)-12-oxophyto-10,15-dienoate + NADPH+ H(+).
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-190308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
12-oxophytodienoate reductase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 44.63 KDa
Source organism: Solanum lycopersicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9FEW9 (Residues: 1-396; Coverage: 100%)
Gene name: OPR3
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21
Unit cell:
a: 49.38Å b: 92.1Å c: 89.96Å
α: 90° β: 99.11° γ: 90°
R-values:
R R work R free
0.165 0.164 0.184
Expression system: Escherichia coli BL21(DE3)