› 8b3f

Electron Microscopy
3.1Å resolution

Pol II-CSB-CSA-DDB1-ELOF1

Released:
DOI: 10.2210/pdb8b3f/pdb
PDB entry 8b3f coloured by chain, front view.
PDB entry 8b3f coloured by chain, side view.
PDB entry 8b3f coloured by chain, top view.
Source organisms:
Entry authors: Kokic G, Cramer P
Related structures: EMD-15826

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero nonadecamer (preferred)
PDBe Complex ID:
PDB-CPX-269368 (preferred)
Entry contents:
16 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (19 distinct):
DNA-directed RNA polymerase subunit Chain: A
Molecule details ›
Chain: A
Length: 1970 amino acids
Theoretical weight: 217.45 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: A0A8D1DPV6 (Residues: 1-1970; Coverage: 100%)
Gene name: POLR2A
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 1167 amino acids
Theoretical weight: 133.2 KDa
Source organism: Sus scrofa domesticus
UniProt:
  • Canonical: I3LGP4 (Residues: 134-1300; Coverage: 90%)
Gene name: POLR2B
Sequence domains:
DNA-directed RNA polymerase II subunit RPB3 Chain: C
DNA-directed RNA polymerase II subunit RPB4 Chain: D
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerase subunit Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein Chain: K
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Transcription elongation factor 1 homolog Chain: M
DNA excision repair protein ERCC-8 Chain: a
DNA excision repair protein ERCC-6 Chain: b
DNA damage-binding protein 1 Chain: d
NTS Chain: N
TS Chain: T
RNA Chain: P

Ligands and Environments

2 bound ligands:
Ligand ZN 9 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.1Å
Relevant EMDB volumes: EMD-15826
Expression systems:
  • Escherichia coli
  • Trichoplusia ni

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