› 8b41

Electron Microscopy
3.8Å resolution

Structure of heteromeric LRRC8A/C (1:1 co-transfected) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1

Released:
DOI: 10.2210/pdb8b41/pdb
PDB entry 8b41 coloured by chain, front view.
PDB entry 8b41 coloured by chain, side view.
PDB entry 8b41 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a volume-regulated heteromeric LRRC8A/C channel.
Rutz S, Deneka D, Dittmann A, Sawicka M, Dutzler R
OpenAccess logo Nat Struct Mol Biol (2022)
PMID: 36522427
Related structures: EMD-15836

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-275025 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Volume-regulated anion channel subunit LRRC8A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 817 amino acids
Theoretical weight: 95.06 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q80WG5 (Residues: 2-810; Coverage: 100%)
Gene names: Lrrc8, Lrrc8a, Swell1
Sequence domains:
Volume-regulated anion channel subunit LRRC8C Chains: E, F
Molecule details ›
Chains: E, F
Length: 811 amino acids
Theoretical weight: 93.47 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8R502 (Residues: 2-803; Coverage: 100%)
Gene names: Ad158, Fad158, Lrrc8c
Sequence domains:
Synthetic nanobody Sb1 Chains: G, H, I, J

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-15836
Expression systems:
  • Homo sapiens
  • Escherichia coli MC1061

Quick links

Citations

2 review citations

Physiology of the volume-sensitive/regulatory anion channel VSOR/VRAC. Part 1: from its discovery and phenotype characterization to the molecular entity identification.
Okada Y. (2024)
1 more

2 mentions without citation

Structure of a volume-regulated heteromeric LRRC8A/C channel.
Rutz et al. (2023)
1 more