› 8b48

X-ray diffraction
2.65Å resolution

Structure of Lentithecium fluviatile carbohydrate esterase from the CE15 family (LfCE15C)

Released:
DOI: 10.2210/pdb8b48/pdb
PDB entry 8b48 coloured by chain, front view.
PDB entry 8b48 coloured by chain, side view.
PDB entry 8b48 coloured by chain, top view.
Source organism: Lentithecium fluviatile
Primary publication:
Structural and functional investigation of a fungal member of carbohydrate esterase family 15 with potential specificity for rare xylans.
Mazurkewich S, Scholzen KC, Brusch RH, Poulsen JCN, Theibich Y, Hüttner S, Olsson L, Larsbrink J, Lo Leggio L
Acta Crystallogr D Struct Biol 79 545-555 (2023)
PMID: 37227091

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217181 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Carbohydrate esterase family 15 protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 394 amino acids
Theoretical weight: 43.23 KDa
Source organism: Lentithecium fluviatile
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A6G1IIU9 (Residues: 17-387; Coverage: 100%)
Gene name: K458DRAFT_349146

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand FMT 2 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P1
Unit cell:
a: 70.74Å b: 79.57Å c: 86.01Å
α: 113.322° β: 98.531° γ: 94.44°
R-values:
R R work R free
0.241 0.238 0.298
Expression system: Komagataella pastoris

Quick links

Citations

1 mention without citation

Structural and functional investigation of a fungal member of carbohydrate esterase family 15 with potential specificity for rare xylans.
Mazurkewich et al. (2023)