› 8b8v

X-ray diffraction
2.3Å resolution

Crystal structure of the Rabies virus RNA free nucleoprotein- phosphoprotein complex

Released:
DOI: 10.2210/pdb8b8v/pdb
Model geometry
Fit model/data
PDB entry 8b8v coloured by chain, front view.
PDB entry 8b8v coloured by chain, side view.
PDB entry 8b8v coloured by chain, top view.
Source organism: Rabies virus CVS-11
Primary publication:
Structure and Dynamics of the Unassembled Nucleoprotein of Rabies Virus in Complex with Its Phosphoprotein Chaperone Module.
Gérard FCA, Bourhis JM, Mas C, Branchard A, Vu DD, Varhoshkova S, Leyrat C, Jamin M
OpenAccess logo Viruses 14 (2022)
PMID: 36560817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-273380 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleoprotein Chain: A
Molecule details ›
Chain: A
Length: 427 amino acids
Theoretical weight: 48.21 KDa
Source organism: Rabies virus CVS-11
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8JXF6 (Residues: 24-450; Coverage: 95%)
Gene name: N
Sequence domains: Rhabdovirus nucleocapsid protein
Phosphoprotein Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.5 KDa
Source organism: Rabies virus CVS-11
Expression system: Escherichia coli
UniProt:
  • Canonical: P22363 (Residues: 1-68; Coverage: 23%)
Gene name: P

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 39.571Å b: 73.617Å c: 154.361Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.227
Expression system: Escherichia coli

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Citations

2 review citations

Negative and ambisense RNA virus ribonucleocapsids: more than protective armor.
Sabsay et al. (2023)
1 more

2 mentions without citation

Structure and Dynamics of the Unassembled Nucleoprotein of Rabies Virus in Complex with Its Phosphoprotein Chaperone Module.
Gérard et al. (2022)
1 more