› 8beh

Electron Microscopy
2.29Å resolution

Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CI membrane tip)

Released:
DOI: 10.2210/pdb8beh/pdb
PDB entry 8beh coloured by chain, front view.
PDB entry 8beh coloured by chain, side view.
PDB entry 8beh coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Cryo-EM structure of the respiratory I + III2 supercomplex from Arabidopsis thaliana at 2 Å resolution.
Klusch N, Dreimann M, Senkler J, Rugen N, Kühlbrandt W, Braun HP
OpenAccess logo Nat Plants (2022)
PMID: 36585502
Related structures: EMD-16003

Function and Biology Details

Reaction catalysed: 
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-130279 (preferred)
Entry contents:
13 distinct polypeptide molecules
Macromolecules (13 distinct):
NADH-ubiquinone oxidoreductase chain 5 Chain: L
Molecule details ›
Chain: L
Length: 669 amino acids
Theoretical weight: 74.5 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: P29388 (Residues: 1-669; Coverage: 100%)
Gene names: AtMg00060/AtMg00513/AtMg00665, NAD5, NAD5C, ND5
Sequence domains:
NADH-ubiquinone oxidoreductase chain 4 Chain: M
Molecule details ›
Chain: M
Length: 495 amino acids
Theoretical weight: 56 KDa
Source organism: Arabidopsis thaliana
UniProt:
  • Canonical: P93313 (Residues: 1-495; Coverage: 100%)
Gene names: AtMg00580, NAD4, ND4
Sequence domains: NADH:quinone oxidoreductase/Mrp antiporter, TM
Acyl carrier protein 1, mitochondrial Chain: T
Transmembrane protein Chain: c
ESSS subunit of NADH:ubiquinone oxidoreductase (Complex I) protein Chain: g
NADH-ubiquinone oxidoreductase Chain: i
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 Chain: j
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-A Chain: k
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Chain: l
Neuronal acetylcholine receptor subunit alpha-5 Chain: m
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 Chain: n
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 Chain: o
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B Chain: p

Ligands and Environments

5 bound ligands:
Ligand 3PH 3 x 3PH
Ligand PGT 1 x PGT
Ligand PTY 1 x PTY
Ligand PC7 1 x PC7
Ligand 8Q1 1 x 8Q1
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.29Å
Relevant EMDB volumes: EMD-16003

Quick links

Citations

3 review citations

An evolving view of complex II-noncanonical complexes, megacomplexes, respiration, signaling, and beyond.
Iverson et al. (2023)
2 more