› 8bf3

X-ray diffraction
1.19Å resolution

Crystal structure of feruloyl esterase wtsFae1B in complex with xylobiose

Released:
DOI: 10.2210/pdb8bf3/pdb
PDB entry 8bf3 coloured by chain, front view.
PDB entry 8bf3 coloured by chain, side view.
PDB entry 8bf3 coloured by chain, top view.
Source organism: uncultured bacterium
Entry author: Wilkens C

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glycoside hydrolase family 13 N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.01 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A5S8WFA0 (Residues: 1-386; Coverage: 100%)
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
2 bound ligands:
Ligand EDO 14 x EDO
Ligand XYP 1 x XYP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P212121
Unit cell:
a: 65.58Å b: 99.35Å c: 132.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 0.117 0.138
Expression system: Escherichia coli

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