› 8bna

X-ray diffraction
2.2Å resolution

BINDING OF HOECHST 33258 TO THE MINOR GROOVE OF B-DNA

Released:
DOI: 10.2210/pdb8bna/pdb
PDB entry 8bna coloured by chain, front view.
PDB entry 8bna coloured by chain, side view.
PDB entry 8bna coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Binding of Hoechst 33258 to the minor groove of B-DNA.
Pjura PE, Grzeskowiak K, Dickerson RE
J Mol Biol 197 257-71 (1987)
PMID: 2445998

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122464 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand HT 1 x HT
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 25.04Å b: 40.33Å c: 65.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 not available not available
Expression system: Not provided

Quick links

Citations

15 review citations

DNA as a target for anticancer compounds: methods to determine the mode of binding and the mechanism of action.
Palchaudhuri et al. (2007)
14 more

7 mentions without citation

Structure-based DNA-targeting strategies with small molecule ligands for drug discovery.
Sheng et al. (2013)
6 more