› 8boz

X-ray diffraction
3.6Å resolution

structure of the Adherent-Invasive Escherichia coli Tle3/Tli3 T6SS effector/immunity complex

Released:
DOI: 10.2210/pdb8boz/pdb
PDB entry 8boz coloured by chain, front view.
PDB entry 8boz coloured by chain, side view.
PDB entry 8boz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3/Tli3 T6SS Effector/Immunity Complex Determined Using an AlphaFold2 Predicted Model.
Le TTH, Kellenberger C, Boyer M, Santucci P, Flaugnatti N, Cascales E, Roussel A, Canaan S, Journet L, Cambillau C
Int J Mol Sci 24 (2023)
PMID: 36675258

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226562 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transmembrane protein Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 658 amino acids
Theoretical weight: 73.99 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2VAD3 (Residues: 1-658; Coverage: 100%)
Gene name: c3395
Lipoprotein Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 274 amino acids
Theoretical weight: 30.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2VA42 (Residues: 1-274; Coverage: 100%)
Gene name: c3394

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 67.86Å b: 449.07Å c: 116.23Å
α: 90° β: 93.18° γ: 90°
R-values:
R R work R free
0.234 0.233 0.268
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Activity and Crystal Structure of the Adherent-Invasive Escherichia coli Tle3/Tli3 T6SS Effector/Immunity Complex Determined Using an AlphaFold2 Predicted Model.
Le et al. (2023)