› 8cu0

X-ray diffraction
1.74Å resolution

12-mer DNA structure of ExBIM bound to RNaseH -modified DDD

Released:
DOI: 10.2210/pdb8cu0/pdb
Model geometry
Fit model/data
PDB entry 8cu0 coloured by chain, front view.
PDB entry 8cu0 coloured by chain, side view.
PDB entry 8cu0 coloured by chain, top view.
Source organisms:
Primary publication:
Conformation and Pairing Properties of an O6-Methyl-2'-deoxyguanosine-Directed Benzimidazole Nucleoside Analog in Duplex DNA.
Kellum AH, Pallan PS, Nilforoushan A, Sturla SJ, Stone MP, Egli M
Chem Res Toxicol (2022)
PMID: 35973057

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122483 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Ribonuclease H Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 142 amino acids
Theoretical weight: 16.33 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KEI9 (Residues: 59-196; Coverage: 70%)
Gene names: BH0863, rnhA
DNA (5'-D(*CP*GP*GP*GP*CP*AP*TP*GP*(OWR)P*CP*CP*G)-3') Chains: D, E
Molecule details ›
Chains: D, E
Length: 12 nucleotides
Theoretical weight: 3.72 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand EDO 5 x EDO
Ligand GOL 3 x GOL
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 36.894Å b: 89.403Å c: 73.89Å
α: 90° β: 101.42° γ: 90°
R-values:
R R work R free
0.204 0.202 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 mention without citation

Design and Production of a New FeCoNiCrAlCu High-Entropy Alloy: Influence of Powder Production Method on Sintering.
Reverte et al. (2021)