› 8cuy

Electron Microscopy
2.4Å resolution

ACP1-KS-AT domains of mycobacterial Pks13

Released:
DOI: 10.2210/pdb8cuy/pdb
PDB entry 8cuy coloured by chain, front view.
PDB entry 8cuy coloured by chain, side view.
PDB entry 8cuy coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structure and dynamics of the essential endogenous mycobacterial polyketide synthase Pks13.
Kim SK, Dickinson MS, Finer-Moore J, Guan Z, Kaake RM, Echeverria I, Chen J, Pulido EH, Sali A, Krogan NJ, Rosenberg OS, Stroud RM
Nat Struct Mol Biol (2023)
PMID: 36782050
Related structures: EMD-27002

Function and Biology Details

Reaction catalysed: 
Propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH = 6-deoxyerythronolide B + 7 CoA + 6 CO(2) + H(2)O + 6 NADP(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125245 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase PKS13 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1816 amino acids
Theoretical weight: 194.67 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: I7FMV0 (Residues: 26-1841; Coverage: 99%)
Gene names: MSMEI_6224, pks13
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand UNL 4 x UNL
Ligand PNS 1 x PNS
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.4Å
Relevant EMDB volumes: EMD-27002

Quick links

Citations

1 review citation

Mycobacterium tuberculosis: Pathogenesis and therapeutic targets.
Yang et al. (2023)
2 other articles

2 mentions without citation

Structure and dynamics of the essential endogenous mycobacterial polyketide synthase Pks13.
Kim et al. (2023)
1 more