› 8cz8

X-ray diffraction
2.6Å resolution

Novel Anti-Mesothelin Antibodies Enable Crystallography of the Intact Mesothelin Ectodo- main and Engineering of Potent, T cell-engaging Bispecific Therapeutics

Released:
DOI: 10.2210/pdb8cz8/pdb
Model geometry
Fit model/data
PDB entry 8cz8 coloured by chain, front view.
PDB entry 8cz8 coloured by chain, side view.
PDB entry 8cz8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Novel mesothelin antibodies enable crystallography of the intact mesothelin ectodomain and engineering of potent, T cell-engaging bispecific therapeutics
Lin I, Rupert PB, Pilat K, Ruff RO, Friend DJ, Chan MK, Clarke M, Hoffstrom BG, Carter J, Meshinchi S, Bandaranayake AD, Mehlin C, Olson JM, Strong RK, Correnti CE
Front. Drug Discov. 3 (2023)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274525 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
scFv Anetumab Chains: A, C
Molecule details ›
Chains: A, C
Length: 247 amino acids
Theoretical weight: 25.53 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Mesothelin, cleaved form Chains: E, M
Molecule details ›
Chains: E, M
Length: 159 amino acids
Theoretical weight: 17.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13421 (Residues: 434-590; Coverage: 26%)
Gene names: MPF, MSLN
scFv of A12 anti-mesothelin antibody Chains: B, D

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P32
Unit cell:
a: 83.795Å b: 83.795Å c: 230.45Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.224
Expression system: Homo sapiens

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