› 8d1d

X-ray diffraction
1.42Å resolution

PROSS PETase

Released:
DOI: 10.2210/pdb8d1d/pdb
PDB entry 8d1d coloured by chain, front view.
PDB entry 8d1d coloured by chain, side view.
PDB entry 8d1d coloured by chain, top view.
Source organism: synthetic construct
Entry authors: Vongsouthi V, Jackson CJ, Tan L

Function and Biology Details

Reaction catalysed: 
(Ethylene terephthalate)(n) + H(2)O = (ethylene terephthalate)(n-1) + ethylene terephthalate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(ethylene terephthalate) hydrolase Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 265 amino acids
Theoretical weight: 27.73 KDa
Source organism: synthetic construct
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A0K8P6T7 (Residues: 32-290; Coverage: 99%)
Gene name: ISF6_4831
Sequence domains: Dienelactone hydrolase family

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 136.586Å b: 159.173Å c: 45.043Å
α: 90° β: 90.07° γ: 90°
R-values:
R R work R free
0.2 0.198 0.234
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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