8d3l

Electron Microscopy
3.49Å resolution

Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/PAM prespacer

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-281289 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
CRISPR-associated endonuclease Cas1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 343 amino acids
Theoretical weight: 39.39 KDa
Source organism: Halalkalibacterium halodurans C-125
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KFX9 (Residues: 1-343; Coverage: 100%)
Gene names: BH0341, cas1
Sequence domains: CRISPR associated protein Cas1
CRISPR-associated endonuclease Cas2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 98 amino acids
Theoretical weight: 11.04 KDa
Source organism: Halalkalibacterium halodurans C-125
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KFX8 (Residues: 1-96; Coverage: 100%)
Gene names: BH0342, cas2
Sequence domains: CRISPR associated protein Cas2
CRISPR-associated exonuclease Cas4 Chains: I, J
PAM/PAM strand 2 Chain: G
PAM/PAM strand 1 Chain: H

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.49Å
Relevant EMDB volumes: EMD-27159
Expression system: Escherichia coli BL21(DE3)