› 8daj

X-ray diffraction
1.2Å resolution

Structure and Biochemistry of a Promiscuous Thermophilic Polyhydroxybutyrate Depolymerase from Lihuaxuella thermophilia

Released:
DOI: 10.2210/pdb8daj/pdb
PDB entry 8daj coloured by chain, front view.
PDB entry 8daj coloured by chain, side view.
PDB entry 8daj coloured by chain, top view.
Source organism: Lihuaxuella thermophila
Primary publication:
Bioplastic degradation by a polyhydroxybutyrate depolymerase from a thermophilic soil bacterium.
Thomas GM, Quirk S, Huard DJE, Lieberman RL
Protein Sci 31 e4470 (2022)
PMID: 36222314

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Esterase, PHB depolymerase family Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 32.44 KDa
Source organism: Lihuaxuella thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1H8IKU3 (Residues: 34-333; Coverage: 90%)
Gene name: SAMN05444955_11823
Sequence domains: Esterase PHB depolymerase

Ligands and Environments

2 bound ligands:
Ligand EDO 10 x EDO
Ligand IPA 4 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 45.51Å b: 45.51Å c: 211.26Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.163 0.177
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Autophagic Regulation of Adipogenesis Through TP53INP2: Insights from In Silico and In Vitro Analysis.
Sekar et al. (2024)
1 more

1 mention without citation

Bioplastic degradation by a polyhydroxybutyrate depolymerase from a thermophilic soil bacterium.
Thomas et al. (2022)