› 8db3

X-ray diffraction
2.9Å resolution

Crystal structure of KaiC with truncated C-terminal coiled-coil domain

Released:
DOI: 10.2210/pdb8db3/pdb
Model geometry
Fit model/data
PDB entry 8db3 coloured by chain, front view.
PDB entry 8db3 coloured by chain, side view.
PDB entry 8db3 coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
From primordial clocks to circadian oscillators.
Pitsawong W, Pádua RAP, Grant T, Hoemberger M, Otten R, Bradshaw N, Grigorieff N, Kern D
OpenAccess logo Nature (2023)
PMID: 36949197

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-256283 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Circadian clock protein KaiC Chains: A, C
Molecule details ›
Chains: A, C
Length: 490 amino acids
Theoretical weight: 53.69 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
Circadian clock protein KaiC Chain: B
Molecule details ›
Chain: B
Length: 490 amino acids
Theoretical weight: 53.77 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand ADP 6 x ADP
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 94.06Å b: 197.28Å c: 150.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.236 0.295
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The inner workings of an ancient biological clock.
Fang et al. (2024)
1 more

1 mention without citation

From primordial clocks to circadian oscillators.
Pitsawong et al. (2023)