› 8db8

X-ray diffraction
2.21Å resolution

Adenosine/guanosine nucleoside hydrolase bound to ImH

Released:
DOI: 10.2210/pdb8db8/pdb
PDB entry 8db8 coloured by chain, front view.
PDB entry 8db8 coloured by chain, side view.
PDB entry 8db8 coloured by chain, top view.
Source organism: Trichomonas vaginalis
Primary publication:
Structure-Guided Insight into the Specificity and Mechanism of a Parasitic Nucleoside Hydrolase.
Muellers SN, Nyitray MM, Reynarowych N, Saljanin E, Benzie AL, Schoenfeld AR, Stockman BJ, Allen KN
Biochemistry (2022)
PMID: 35994320

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106836 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine/uridine-preferring nucleoside hydrolase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 304 amino acids
Theoretical weight: 33.48 KDa
Source organism: Trichomonas vaginalis
Expression system: Escherichia coli
UniProt:
  • Canonical: A2EYV3 (Residues: 1-304; Coverage: 100%)
Gene name: TVAG_213720
Sequence domains: Inosine-uridine preferring nucleoside hydrolase

Ligands and Environments

2 bound ligands:
Ligand IMH 2 x IMH
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P1
Unit cell:
a: 51.025Å b: 76.48Å c: 86.636Å
α: 75.479° β: 81.672° γ: 86.504°
R-values:
R R work R free
0.223 0.221 0.266
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

A riboside hydrolase that salvages both nucleobases and nicotinamide in the auxotrophic parasite Trichomonas vaginalis.
Patrone et al. (2023)
1 more

1 mention without citation

Ribonucleoside Hydrolases-Structure, Functions, Physiological Role and Practical Uses.
Shaposhnikov et al. (2023)