› 8dba

X-ray diffraction
3.5Å resolution

Crystal structure of dodecameric KaiC

Released:
DOI: 10.2210/pdb8dba/pdb
PDB entry 8dba coloured by chain, front view.
PDB entry 8dba coloured by chain, side view.
PDB entry 8dba coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
From primordial clocks to circadian oscillators.
Pitsawong W, Pádua RAP, Grant T, Hoemberger M, Otten R, Bradshaw N, Grigorieff N, Kern D
Nature (2023)
PMID: 36949197

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110854 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
non-specific serine/threonine protein kinase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 568 amino acids
Theoretical weight: 62.58 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9KWX8 (Residues: 1-566; Coverage: 100%)
Gene name: RSKD131_4430
Sequence domains: KaiC

Ligands and Environments

2 bound ligands:
Ligand ADP 24 x ADP
Ligand MG 23 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P1
Unit cell:
a: 105.11Å b: 136.09Å c: 146.2Å
α: 93.13° β: 94.43° γ: 108.09°
R-values:
R R work R free
0.236 0.236 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Uncoupled redox stress: how a temporal misalignment of redox-regulated processes and circadian rhythmicity exacerbates the stressed state.
Clark et al. (2023)
2 other articles

1 mention without citation

From primordial clocks to circadian oscillators.
Pitsawong et al. (2023)