› 8e19

X-ray diffraction
2.03Å resolution

Crystal structure of TnmK1 complexed with TNM H

Released:
DOI: 10.2210/pdb8e19/pdb
PDB entry 8e19 coloured by chain, front view.
PDB entry 8e19 coloured by chain, side view.
PDB entry 8e19 coloured by chain, top view.
Source organism: Streptomyces sp. CB03234
Primary publication:
Intramolecular C-C Bond Formation Links Anthraquinone and Enediyne Scaffolds in Tiancimycin Biosynthesis.
Gui C, Kalkreuter E, Liu YC, Adhikari A, Teijaro CN, Yang D, Chang C, Shen B
J Am Chem Soc (2022)
PMID: 36279548

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102864 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 484 amino acids
Theoretical weight: 52.2 KDa
Source organism: Streptomyces sp. CB03234
Expression system: Streptomyces lividans
UniProt:
  • Canonical: A0A125SA14 (Residues: 1-484; Coverage: 100%)
Gene names: AMK26_32035, tnmK1
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

3 bound ligands:
Ligand U8L 1 x U8L
Ligand EPE 1 x EPE
Ligand SIN 1 x SIN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P41212
Unit cell:
a: 85.843Å b: 85.843Å c: 129.317Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.206
Expression system: Streptomyces lividans

Quick links

Citations

2 citation in other articles

A discrete intermediate for the biosynthesis of both the enediyne core and the anthraquinone moiety of enediyne natural products.
Bhardwaj et al. (2023)
1 more

2 mentions without citation

Near-UV electroluminescence in unipolar-doped, bipolar-tunneling GaN/AlN heterostructures.
Growden et al. (2018)
1 more