› 8e5k

Electron Microscopy
4.2Å resolution

Escherichia coli Rho-dependent transcription pre-termination complex containing 21 nt long RNA spacer, Mg-ADP-BeF3, and NusG; TEC part

Released:
DOI: 10.2210/pdb8e5k/pdb
PDB entry 8e5k coloured by chain, front view.
PDB entry 8e5k coloured by chain, side view.
PDB entry 8e5k coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of Rho-dependent transcription termination.
Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH
Nature (2023)
PMID: 36697824
Related structures: EMD-27913

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-122543 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit beta Chain: A
Molecule details ›
Chain: A
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: B
Molecule details ›
Chain: B
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit alpha Chains: C, D
Molecule details ›
Chains: C, D
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
Transcription termination/antitermination protein NusG Chain: F
Molecule details ›
Chain: F
Length: 181 amino acids
Theoretical weight: 20.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFG0 (Residues: 1-181; Coverage: 100%)
Gene names: JW3945, b3982, nusG
Sequence domains:
NT DNA Chain: 5
Molecule details ›
Chain: 5
Length: 60 nucleotides
Theoretical weight: 18.38 KDa
T DNA Chain: 6
Molecule details ›
Chain: 6
Length: 60 nucleotides
Theoretical weight: 18.45 KDa
RNA with 21 nt long spacer Chain: 7
Molecule details ›
Chain: 7
Length: 38 nucleotides
Theoretical weight: 11.99 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-27913
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Knowing when to stop: Transcription termination on protein-coding genes by eukaryotic RNAPII.
Rodríguez-Molina et al. (2023)
6 other articles

1 mention without citation

Structural basis of Rho-dependent transcription termination.
Molodtsov et al. (2023)