› 8e6n

X-ray diffraction
2.4Å resolution

X-ray structure of the Deinococcus radiodurans Nramp/MntH divalent transition metal transporter G223W mutant in an outward-open, manganese-bound state

Released:
DOI: 10.2210/pdb8e6n/pdb
PDB entry 8e6n coloured by chain, front view.
PDB entry 8e6n coloured by chain, side view.
PDB entry 8e6n coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
High-resolution structures with bound Mn2+ and Cd2+ map the metal import pathway in an Nramp transporter.
Ray S, Berry SP, Wilson EA, Zhang CH, Shekhar M, Singharoy A, Gaudet R
Elife 12 (2023)
PMID: 37039477

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Divalent metal cation transporter MntH Chain: A
Molecule details ›
Chain: A
Length: 411 amino acids
Theoretical weight: 44.48 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RTP8 (Residues: 35-436; Coverage: 92%)
Gene names: DR_1709, mntH
Sequence domains: Natural resistance-associated macrophage protein-like

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand OLC 18 x OLC
Ligand PEG 4 x PEG
Ligand SPD 2 x SPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 105.758Å b: 80.389Å c: 51.754Å
α: 90° β: 94.72° γ: 90°
R-values:
R R work R free
0.226 0.224 0.271
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structures and coordination chemistry of transporters involved in manganese and iron homeostasis.
Ray et al. (2023)
3 other articles

2 mentions without citation

Membrane Transporters Involved in Iron Trafficking: Physiological and Pathological Aspects.
Pasquadibisceglie et al. (2023)
1 more