› 8eb5

X-ray diffraction
2.5Å resolution

Tandem of Hermes transposase BED domain in complex with the quasi palindrome of its transposon left-end

Released:
DOI: 10.2210/pdb8eb5/pdb
Model geometry
Fit model/data
PDB entry 8eb5 coloured by chain, front view.
PDB entry 8eb5 coloured by chain, side view.
PDB entry 8eb5 coloured by chain, top view.
Source organism: Musca domestica
Primary publication:
Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding.
Lannes L, Furman CM, Hickman AB, Dyda F
OpenAccess logo Nat Commun 14 4470 (2023)
PMID: 37491363

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-270383 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
BED-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 78 amino acids
Theoretical weight: 9.15 KDa
Source organism: Musca domestica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q25442 (Residues: 1-78; Coverage: 13%)
Hermes transposon left-end subterminal repeats 1 and 2 Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.2 KDa
Source organism: Musca domestica
Expression system: Not provided
Hermes transposon left-end subterminal repeats 1 and 2 Chain: D

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6522
Unit cell:
a: 55.271Å b: 55.271Å c: 212.573Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.231 0.238
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 mention without citation

Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding.
Lannes et al. (2023)