› 8ee3

X-ray diffraction
1.55Å resolution

F93Y Horse Liver Alcohol Dehydrogenase in Complex with NADH and N-Cyclohexylformamide

Released:
DOI: 10.2210/pdb8ee3/pdb
Model geometry
Fit model/data
PDB entry 8ee3 coloured by chain, front view.
PDB entry 8ee3 coloured by chain, side view.
PDB entry 8ee3 coloured by chain, top view.
Source organism: Equus caballus
Entry authors: Zheng C, Boxer SG

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase E chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 377 amino acids
Theoretical weight: 40.05 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
2 bound ligands:
Ligand ZN 4 x ZN
Ligand CXF 2 x CXF
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: P1
Unit cell:
a: 44.328Å b: 50.681Å c: 92.93Å
α: 92.49° β: 102.96° γ: 109°
R-values:
R R work R free
0.164 0.163 0.183
Expression system: Escherichia coli

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