› 8ek9

X-ray diffraction
1.4Å resolution

Crystal structure of the class A carbapenemase CRH-1 in complex with avibactam at 1.4 Angstrom resolution

Released:
DOI: 10.2210/pdb8ek9/pdb
Model geometry
Fit model/data
PDB entry 8ek9 coloured by chain, front view.
PDB entry 8ek9 coloured by chain, side view.
PDB entry 8ek9 coloured by chain, top view.
Source organism: Chromobacterium haemolyticum
Primary publication:
Biochemical and Structural Characterization of CRH-1, a Carbapenemase from Chromobacterium haemolyticum Related to KPC β-Lactamases.
Brunetti F, Ghiglione B, Gudeta DD, Gutkind G, Guardabassi L, Klinke S, Power P
Antimicrob Agents Chemother e0006123 (2023)
PMID: 37272821

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 28.69 KDa
Source organism: Chromobacterium haemolyticum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1W0D7S2 (Residues: 21-290; Coverage: 100%)
Gene name: B0T45_03570
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NXL 1 x NXL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 36.5Å b: 73.79Å c: 51.53Å
α: 90° β: 92.64° γ: 90°
R-values:
R R work R free
0.196 0.194 0.219
Expression system: Escherichia coli BL21(DE3)

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Citations

1 mention without citation

Biochemical and Structural Characterization of CRH-1, a Carbapenemase from Chromobacterium haemolyticum Related to KPC β-Lactamases.
Brunetti et al. (2023)