› 8eka

Electron Microscopy
3.7Å resolution

Cryo-EM structure of a potent anti-malarial antibody L9 in complex with Plasmodium falciparum circumsporozoite protein (PfCSP)(class 2)

Released:
DOI: 10.2210/pdb8eka/pdb
PDB entry 8eka coloured by chain, front view.
PDB entry 8eka coloured by chain, side view.
PDB entry 8eka coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of anti-malarial antibody L9 with circumsporozoite protein reveal trimeric L9 association and complete 27-residue epitope.
Tripathi P, Bender MF, Lei H, Da Silva Pereira L, Shen CH, Bonilla B, Dillon M, Ou L, Pancera M, Wang LT, Zhang B, Batista FD, Idris AH, Seder RA, Kwong PD
Structure 31 480-491.e4 (2023)
PMID: 36931276
Related structures: EMD-28196

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-274981 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Circumsporozoite protein Chain: P
Molecule details ›
Chain: P
Length: 355 amino acids
Theoretical weight: 37.81 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7K740 (Residues: 21-375; Coverage: 94%)
Gene names: CSP, PF3D7_0304600
Sequence domains: Thrombospondin type 1 domain
L9 Fab heavy chain Chains: A, C, H
Molecule details ›
Chains: A, C, H
Length: 227 amino acids
Theoretical weight: 24.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
L9 Fab light chain Chains: B, D, L

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.7Å
Relevant EMDB volumes: EMD-28196
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Monoclonal antibodies to the circumsporozoite proteins as an emerging tool for malaria prevention.
Wang et al. (2024)
1 other article

1 mention without citation

Cryo-EM structures of anti-malarial antibody L9 with circumsporozoite protein reveal trimeric L9 association and complete 27-residue epitope.
Tripathi et al. (2023)