› 8ekg

X-ray diffraction
2.65Å resolution

MHETase variant Thr159Val, Met192Tyr, Tyr252Phe, Tyr503Trp

Released:
DOI: 10.2210/pdb8ekg/pdb
PDB entry 8ekg coloured by chain, front view.
PDB entry 8ekg coloured by chain, side view.
PDB entry 8ekg coloured by chain, top view.
Source organism: Ideonella sakaiensis
Entry authors: Saunders JW, Frkic RL, Jackson CJ

Function and Biology Details

Reaction catalysed: 
Ethylene terephthalate + H(2)O = terephthalate + ethylene glycol
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mono(2-hydroxyethyl) terephthalate hydrolase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 592 amino acids
Theoretical weight: 62.37 KDa
Source organism: Ideonella sakaiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0K8P8E7 (Residues: 20-603; Coverage: 100%)
Gene name: ISF6_0224
Sequence domains: Tannase and feruloyl esterase

Ligands and Environments

4 bound ligands:
Ligand CA 6 x CA
Ligand PEG 1 x PEG
Ligand EDO 11 x EDO
Ligand SO4 4 x SO4
1 modified residue:
Ligand SEP 6 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 63.967Å b: 120.514Å c: 126.29Å
α: 90.44° β: 95.15° γ: 90.2°
R-values:
R R work R free
0.187 0.184 0.241
Expression system: Escherichia coli

Quick links