› 8eu4

X-ray diffraction
2.1Å resolution

Escherichia coli pyruvate kinase A301S

Released:
DOI: 10.2210/pdb8eu4/pdb
Model geometry
Fit model/data
PDB entry 8eu4 coloured by chain, front view.
PDB entry 8eu4 coloured by chain, side view.
PDB entry 8eu4 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Donovan KA, Coombes D, Dobson RCJ, Cooper TF

Function and Biology Details

Reaction catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase I Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 470 amino acids
Theoretical weight: 50.81 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AD61 (Residues: 1-470; Coverage: 100%)
Gene names: JW1666, b1676, pykF
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 129.334Å b: 74.473Å c: 240.826Å
α: 90° β: 90.04° γ: 90°
R-values:
R R work R free
0.204 0.202 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links