› 8ey4

X-ray diffraction
1.83Å resolution

Contact-dependent growth inhibition toxin-immunity protein complex from E. coli O32:H37

Released:
DOI: 10.2210/pdb8ey4/pdb
Model geometry
Fit model/data
PDB entry 8ey4 coloured by chain, front view.
PDB entry 8ey4 coloured by chain, side view.
PDB entry 8ey4 coloured by chain, top view.
Source organism: Escherichia coli O32:H37
Entry authors: Michalska K, Stols L, Eschenfeldt W, Goulding CW, Hayes CS, Joachimiak A, Midwest Center for Structural Genomics (MCSG), Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269004 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine-rich CPCC domain-containing protein Chain: I
Molecule details ›
Chain: I
Length: 70 amino acids
Theoretical weight: 7.64 KDa
Source organism: Escherichia coli O32:H37
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1Y2XHL0 (Residues: 1-61; Coverage: 100%)
Gene names: B6R15_004523, C0P57_003609, D9E49_27105
Sequence domains: Cysteine-rich CPCC
VENN motif-containing domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.18 KDa
Source organism: Escherichia coli O32:H37
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3ZTX4 (Residues: 2614-2701; Coverage: 3%)
Gene name: LH0189

Ligands and Environments

1 bound ligand:
Ligand FE 1 x FE
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 70.641Å b: 53.66Å c: 38.533Å
α: 90° β: 102.55° γ: 90°
R-values:
R R work R free
0.161 0.157 0.202
Expression system: Escherichia coli BL21(DE3)

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