› 8eyn

X-ray diffraction
1.94Å resolution

Crystal Structure of Human Mitochondrial NADP+ Malic Enzyme 3 in Apo Form

Released:
DOI: 10.2210/pdb8eyn/pdb
Model geometry
Fit model/data
PDB entry 8eyn coloured by chain, front view.
PDB entry 8eyn coloured by chain, side view.
PDB entry 8eyn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Integrative structural and functional analysis of human malic enzyme 3: A potential therapeutic target for pancreatic cancer.
Grell TAJ, Mason M, Thompson AA, Gómez-Tamayo JC, Riley D, Wagner MV, Steele R, Ortiz-Meoz RF, Wadia J, Shaffer PL, Tresadern G, Sharma S, Yu X
OpenAccess logo Heliyon 8 e12392 (2022)
PMID: 36590518

Function and Biology Details

Reaction catalysed: 
(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent malic enzyme, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 568 amino acids
Theoretical weight: 63.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16798 (Residues: 46-604; Coverage: 93%)
Gene name: ME3
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21212
Unit cell:
a: 151.75Å b: 75.13Å c: 118.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Integrative structural and functional analysis of human malic enzyme 3: A potential therapeutic target for pancreatic cancer.
Grell et al. (2022)