› 8f5d

X-ray diffraction
2.56Å resolution

Architecture of the MurE-MurF ligase bacterial cell wall biosynthesis complex

Released:
DOI: 10.2210/pdb8f5d/pdb
Model geometry
Fit model/data
PDB entry 8f5d coloured by chain, front view.
PDB entry 8f5d coloured by chain, side view.
PDB entry 8f5d coloured by chain, top view.
Source organism: Bordetella pertussis 18323
Primary publication:
Architecture and genomic arrangement of the MurE-MurF bacterial cell wall biosynthesis complex.
Shirakawa KT, Sala FA, Miyachiro MM, Job V, Trindade DM, Dessen A
OpenAccess logo Proc Natl Acad Sci U S A 120 e2219540120 (2023)
PMID: 37186837

Function and Biology Details

Reactions catalysed: 
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217151 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multifunctional fusion protein Chain: A
Molecule details ›
Chain: A
Length: 803 amino acids
Theoretical weight: 84.34 KDa
Source organism: Bordetella pertussis 18323
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0T7CQ89 (Residues: 2-802; Coverage: 85%)
Gene names: BN118_3149, murE, murF
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: LNLS SIRIUS BEAMLINE MANACA
Spacegroup: I222
Unit cell:
a: 105.636Å b: 111.106Å c: 219.151Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.249 0.292
Expression system: Escherichia coli

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Citations

2 mentions without citation

Camphor, Artemisinin and Sumac Phytochemicals as inhibitors against COVID-19: Computational approach.
Belhassan et al. (2021)
1 more