8f7w

Electron Microscopy
3.19Å resolution

Gi bound kappa-opioid receptor in complex with dynorphin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-279421 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Kappa-type opioid receptor Chain: R
Molecule details ›
Chain: R
Length: 378 amino acids
Theoretical weight: 42.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P41145 (Residues: 3-380; Coverage: 100%)
Gene names: OPRK, OPRK1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Dynorphin A(1-8) Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 983 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01213 (Residues: 207-214; Coverage: 3%)
Gene name: PDYN
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
scFv16 Chain: E

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.19Å
Relevant EMDB volumes: EMD-28911
Expression systems:
  • Spodoptera frugiperda
  • Not provided
  • Escherichia coli