› 8ffz

Electron Microscopy
3.8Å resolution

TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene

Released:
DOI: 10.2210/pdb8ffz/pdb
PDB entry 8ffz coloured by chain, front view.
PDB entry 8ffz coloured by chain, side view.
PDB entry 8ffz coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of TFIIIC-dependent RNA polymerase III transcription initiation.
Talyzina A, Han Y, Banerjee C, Fishbain S, Reyes A, Vafabakhsh R, He Y
Mol Cell (2023)
PMID: 37402369
Related structures: EMD-29071

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-218480 (preferred)
Entry contents:
8 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (10 distinct):
Transcription factor IIIA Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 52.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P39933 (Residues: 1-429; Coverage: 100%)
Gene names: P9677.9, PZF1, TFC2, TFIIIA, YPR186C
Sequence domains:
Transcription factor tau 138 kDa subunit Chain: B
Molecule details ›
Chain: B
Length: 1160 amino acids
Theoretical weight: 132.31 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P34111 (Residues: 1-1160; Coverage: 100%)
Gene names: FUN24, TFC3, TSV115, YAL001C
Sequence domains:
Transcription factor tau 131 kDa subunit Chain: C
Molecule details ›
Chain: C
Length: 1025 amino acids
Theoretical weight: 120.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33339 (Residues: 1-1025; Coverage: 100%)
Gene names: PCF1, TFC4, YGR047C
Sequence domains: Tetratricopeptide repeat
Transcription factor tau 95 kDa subunit Chain: D
Molecule details ›
Chain: D
Length: 649 amino acids
Theoretical weight: 73.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32367 (Residues: 1-649; Coverage: 100%)
Gene names: TFC1, YBR0919, YBR123C
Sequence domains:
Transcription factor tau 91 kDa subunit Chain: E
Molecule details ›
Chain: E
Length: 672 amino acids
Theoretical weight: 74.8 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q06339 (Residues: 1-672; Coverage: 100%)
Gene names: TFC6, YDR362C
Transcription factor tau 60 kDa subunit Chain: F
Molecule details ›
Chain: F
Length: 588 amino acids
Theoretical weight: 67.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12308 (Residues: 1-588; Coverage: 100%)
Gene names: TFC8, YPL007C
Sequence domains: Putative zinc-finger of transcription factor IIIC complex
Transcription factor tau 55 kDa subunit Chain: G
Molecule details ›
Chain: G
Length: 435 amino acids
Theoretical weight: 49.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12415 (Residues: 1-435; Coverage: 100%)
Gene names: O3234, TFC7, YOR110W, YOR3234w
Sequence domains:
TATA-box-binding protein; Transcription factor IIIB 70 kDa subunit Chain: H
Molecule details ›
Chain: H
Length: 736 amino acids
Theoretical weight: 82.1 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P29056 (Residues: 1-382, 439-596; Coverage: 91%)
  • Canonical: P13393 (Residues: 61-240; Coverage: 75%)
Gene names: BRF1, BTF1, PCF4, SPT15, TBP1, TDS4, YER148W, YGR246C
Sequence domains:
DNA (151-MER) Chain: I
Molecule details ›
Chain: I
Length: 171 nucleotides
Theoretical weight: 52.64 KDa
Sequence domains: 5S ribosomal RNA
DNA (151-MER) Chain: J
Molecule details ›
Chain: J
Length: 171 nucleotides
Theoretical weight: 52.93 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 9 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-29071
Expression systems:
  • Escherichia coli
  • Saccharomyces cerevisiae

Quick links

Citations

2 citation in other articles

Characterization of Tau95 led to the identification of a four-subunit TFIIIC complex in trypanosomatid parasites.
Mondragón-Rosas et al. (2024)
1 more

1 mention without citation

Structural basis of TFIIIC-dependent RNA Polymerase III transcription initiation.
Talyzina et al. (2023)