› 8fmg

X-ray diffraction
1.79Å resolution

Structure of CBASS Cap5 from Pseudomonas syringae as an activated tetramer with the cyclic dinucleotide 3'2'-c-diAMP ligand (3 tetramers in the AU)

Released:
DOI: 10.2210/pdb8fmg/pdb
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PDB entry 8fmg coloured by chain, front view.
PDB entry 8fmg coloured by chain, side view.
PDB entry 8fmg coloured by chain, top view.
Source organism: Pseudomonas syringae
Primary publication:
Activation of CBASS Cap5 endonuclease immune effector by cyclic nucleotides.
Rechkoblit O, Sciaky D, Kreitler DF, Buku A, Kottur J, Aggarwal AK
Nat Struct Mol Biol (2024)
PMID: 38321146

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-269135 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HNH endonuclease Chains: A, B, C, D, E, F, G, H, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, K, L, M, N
Length: 388 amino acids
Theoretical weight: 42.77 KDa
Source organism: Pseudomonas syringae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2P0QGK5 (Residues: 10-397; Coverage: 98%)
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 12 x ZN
Ligand MG 13 x MG
Ligand Y4F 12 x Y4F
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: C2
Unit cell:
a: 145.192Å b: 80.522Å c: 406.841Å
α: 90° β: 90.62° γ: 90°
R-values:
R R work R free
0.182 0.179 0.225
Expression system: Escherichia coli

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