› 8g0g

X-ray diffraction
2.1Å resolution

Crystal structure of diphtheria toxin H223Q/H257Q double mutant (pH 4.5)

Released:
DOI: 10.2210/pdb8g0g/pdb
Model geometry
Fit model/data
PDB entry 8g0g coloured by chain, front view.
PDB entry 8g0g coloured by chain, side view.
PDB entry 8g0g coloured by chain, top view.
Source organism: Corynebacterium diphtheriae
Primary publication:
Histidine Protonation and Conformational Switching in Diphtheria Toxin Translocation Domain.
Rodnin MV, Vasques-Montes V, Kyrychenko A, Oliveira NFB, Kashipathy MM, Battaile KP, Douglas J, Lovell S, Machuqueiro M, Ladokhin AS
OpenAccess logo Toxins (Basel) 15 (2023)
PMID: 37505680

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-220955 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diphtheria toxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 538 amino acids
Theoretical weight: 58.72 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5PY51 (Residues: 1-536; Coverage: 100%)
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand APU 2 x APU
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P1
Unit cell:
a: 69.36Å b: 69.399Å c: 69.67Å
α: 64.51° β: 76.51° γ: 81.19°
R-values:
R R work R free
0.192 0.19 0.239
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Towards a unifying model for B-cell receptor triggering.
Degn et al. (2024)
1 other article

1 mention without citation

Histidine Protonation and Conformational Switching in Diphtheria Toxin Translocation Domain.
Rodnin et al. (2023)