› 8g1u

Electron Microscopy
2.83Å resolution

Structure of the methylosome-Lsm10/11 complex

Released:
DOI: 10.2210/pdb8g1u/pdb
PDB entry 8g1u coloured by chain, front view.
PDB entry 8g1u coloured by chain, side view.
PDB entry 8g1u coloured by chain, top view.
Source organisms:
Primary publication:
In vitro methylation of the U7 snRNP subunits Lsm11 and SmE by the PRMT5/MEP50/pICln methylosome.
Yang XC, Desotell A, Lin MH, Paige AS, Malinowska A, Sun Y, Aik WS, Dadlez M, Tong L, Dominski Z
RNA (2023)
PMID: 37562960
Related structures: EMD-29677

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-227095 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Protein arginine N-methyltransferase 5 Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 637 amino acids
Theoretical weight: 72.77 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O14744 (Residues: 1-637; Coverage: 100%)
Gene names: HRMT1L5, IBP72, JBP1, PRMT5, SKB1
Sequence domains:
Methylosome protein WDR77 Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 342 amino acids
Theoretical weight: 36.76 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9BQA1 (Residues: 1-342; Coverage: 100%)
Gene names: HKMT1069, MEP50, Nbla10071, WD45, WDR77
Sequence domains: WD domain, G-beta repeat
Methylosome subunit pICln Chains: C, G, K, O
Molecule details ›
Chains: C, G, K, O
Length: 236 amino acids
Theoretical weight: 26.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q61189 (Residues: 1-236; Coverage: 100%)
Gene names: Clci, Clcni, Clns1a
Sequence domains: Regulator of volume decrease after cellular swelling
U7 snRNA-associated Sm-like protein LSm11 Chains: D, H, L, P
Molecule details ›
Chains: D, H, L, P
Length: 360 amino acids
Theoretical weight: 39.57 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P83369 (Residues: 1-360; Coverage: 100%)
Gene name: LSM11

Ligands and Environments

1 bound ligand:
Ligand ADN 4 x ADN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.83Å
Relevant EMDB volumes: EMD-29677
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21(DE3)

Quick links