› 8gat

Electron Microscopy
3Å resolution

Structure of human NDS.1 Fab and 1G01 Fab in complex with influenza virus neuraminidase from A/Indiana/10/2011 (H3N2v), based on consensus cryo-EM map with only Fab 1G01 resolved

Released:
DOI: 10.2210/pdb8gat/pdb
PDB entry 8gat coloured by chain, front view.
PDB entry 8gat coloured by chain, side view.
PDB entry 8gat coloured by chain, top view.
Source organisms:
Primary publication:
Protective human monoclonal antibodies target conserved sites of vulnerability on the underside of influenza virus neuraminidase.
Lederhofer J, Tsybovsky Y, Nguyen L, Raab JE, Creanga A, Stephens T, Gillespie RA, Syeda HZ, Fisher BE, Skertic M, Yap C, Schaub AJ, Rawi R, Kwong PD, Graham BS, McDermott AB, Andrews SF, King NP, Kanekiyo M
Immunity (2024)
PMID: 38430907
Related structures: EMD-29907

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-240455 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Neuraminidase; Vasodilator-stimulated phosphoprotein Chain: A
Molecule details ›
Chain: A
Length: 466 amino acids
Theoretical weight: 51.8 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P50552 (Residues: 337-375; Coverage: 10%)
  • Canonical: G9LQ08 (Residues: 83-469; Coverage: 83%)
Gene names: L998_47825gpNA, NA, VASP
Sequence domains:
Fab 1G01, heavy chain Chain: M
Molecule details ›
Chain: M
Length: 240 amino acids
Theoretical weight: 25.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fab 1G01, light chain Chain: N
Molecule details ›
Chain: N
Length: 216 amino acids
Theoretical weight: 23.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-29907
Expression system: Homo sapiens

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